Anti-GST
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Catalog #
AB9929
Category
Antibodies
Family
Fusion / Marker Proteins
Source
Goat
Application
WB
Reactivity
Transformed cells
Description
Goat polyclonal antibody to GST (Glutathione-S-Transferase). GST is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in GST plasmid expression vectors as a fusion protein with foreign protein.
Alternative names
Glutathione-S-Transferase
Gene Identifier/Accession#
N/A
Form
​Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant protein produced in E. coli.
Antigen Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFPGRLERPHR
Specificity
In lysates of E. coli cells transformed with pGEX plasmid detects a band of 26 kDa by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transformed cells +++ ND ND ND ND

Images

Anti-GST Ab at 1/2,000 dilution; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

300 €

Quantity / Unit Size

600 µg
1.5 mg
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