Anti-Rab5b

Catalog #
AB0012
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to mouse Rab5b. Rab5b belongs to the small GTPase superfamily, Rab family. Rab5b is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
Alternative names
RAB5B, RAS-associated protein RAB5B, ras-related protein Rab-5B antibody.
Gene Identifier/Accession#
ENSG00000111540
Form
Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.
Antigen Sequence
N/A
Specificity
Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b; it does not recognize Rab5a and Rab5c.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:250-1:1,000
IF 1:50-1:250
IHC (F) 1:100-1:500
IHC (P) 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

Images

IHC of mouse pancreas using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:250/DAB detection.
Anti-Rab5b Ab at 1/500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
IHC of mouse retina using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:500/DAB detection.
Anti-Rab5b Ab at 1/500 dilution; 293 cells transfected with GFP-Rab5; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-Rab5b Ab using B6-RPE07 cells at 1/100 dilution;
IHC of mouse stomach using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:500/DAB detection.

320 €

Quantity / Unit Size

800 µg
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