Anti-Rab5b
- Catalog #
- AB0012
- Category
- Antibodies
- Family
- Vesicle Trafficking
- Source
- Goat
- Application
- WB, IHC (F), IHC (P), IF
- Reactivity
- Human, Rat, Mouse, Monkey, Canine
- Description
- Goat polyclonal antibody to mouse Rab5b. Rab5b belongs to the small GTPase superfamily, Rab family. Rab5b is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
- Alternative names
- RAB5B, RAS-associated protein RAB5B, ras-related protein Rab-5B antibody.
- Gene Identifier/Accession#
- ENSG00000111540
- Form
- Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 4.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.
- Antigen Sequence
- N/A
- Specificity
- Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b; it does not recognize Rab5a and Rab5c.
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.
-
Application Values WB 1:250-1:1,000 IF 1:50-1:250 IHC (F) 1:100-1:500 IHC (P) 1:100-1:500 -
Sample WB IHC (F) IHC (P) IF ELISA Human +++ +++ +++ +++ ND Rat +++ +++ +++ +++ ND Mouse +++ +++ +++ +++ ND Canine +++ +++ +++ +++ ND Monkey +++ +++ +++ +++ ND -
IHC of mouse pancreas using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:250/DAB detection. 
Anti-Rab5b Ab at 1/500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution; 
IHC of mouse retina using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:500/DAB detection. 
Anti-Rab5b Ab at 1/500 dilution; 293 cells transfected with GFP-Rab5; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution; 
Immunofluorescence – anti-Rab5b Ab using B6-RPE07 cells at 1/100 dilution; 
IHC of mouse stomach using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b Ab at 1:500/DAB detection.
Usage
Reactivity Chart
External References (1)
Images
320 €
Quantity / Unit Size
800 µg
Add to shopping cart
Shipping
No Shipping