Anti-Rab1b

Catalog #
AB0017
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB, IHC (F), IHC (P)
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to Rab1b. Rab1b belongs to the small GTPase superfamily, Rab family. Rab1b together with Rab1a control vesicle traffic from the endoplasmic reticulum to the Golgi apparatus.
Alternative names
RAB1A, RAB1B, RAS-associated protein RAB1A, ras-related protein Rab-1B, ras-related protein Rab-1A, ras-related protein Rab-1B, YPT1 antibody.
Gene Identifier/Accession#
Gene ID: 81876, ENSG00000174903
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of Rab1b produced in E. coli.
Antigen Sequence
QEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGVPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPASGGCC
Specificity
Detects endogenous levels of Rab1b protein by Western blot in whole cell lysates and transfected cells with GFP-Rab1b cds.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:100-1:500
IHC (P) 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ ND ND
Rat +++ +++ +++ ND ND
Mouse +++ +++ +++ ND ND
Canine +++ +++ +++ ND ND
Monkey +++ +++ +++ ND ND

Images

Anti-Rab1b Ab at 1/1,000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
IHC of human muscle using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1b Ab at 1:250/DAB detection.
IHC of mouse intestine using anti Rab1b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1b Ab at 1:250/DAB detection.
IHC of human skin using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1b Ab at 1:250/DAB detection.
IHC of human skin using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1b Ab at 1:250/DAB detection.

320 €

Quantity / Unit Size

300 µg
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