Anti-CANX

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Catalog #: AB0037
Category: Antibodies
Family: Loading Controls
Source: Goat
Application: WB, IHC (F), IHC (P), IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the Calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Alternative names: Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.
Gene Identifier/Accession#: Gene ID: 821, ENSG00000127022
Form: Polyclonal antibody supplied as a 200 or 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 2.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.
Antigen Sequence: KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:500-1:5,000
IHC (F)	1:200-1:1,000
IHC (P)	1:200-1:1,000
IF	1:50-1:500

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (34)

Amaro A, Sousa D, Sá-Rocha M, et al. Life Sci 2023 Mar	PMID: 36948389
Tavares G, Rosendo-Silva D, Simões F, et al. Int J Mol Sci 2023 Jan	PMID: 36768789
Gil-Cabrerizo P, Saludas L, Felipe Prósper F et al. Int J Pharm 2022 Dec	PMID: 36332831
Alves LS, Marques ARA, Padrao N, et al. J Cell Sci 2021 Sep.	PMID: 34528688
Neves CAF, MSc Thesis University of Aveiro, Portugal 2015
Sivadasan R, PhD Thesis, Wurzburg University 2016
Thieleke-Matos C, Lopes da Silva M, Cabrita-Santos L et al. Cellular Microbiology 2016 Mar	PMID: 26399761
Rodrigues T, Matafome P, Sereno J, et al. Sci Rep 2017 May	PMID: 28490763
Cabral AMD, MSc Thesis, University of Lisbon 2017
Ribeiro STF, PhD Thesis University of Lisbon, Portugal 2017

Images

	Immunofluorescence – anti-CANX Ab in HeLa cells at 1/250 dilution; cells were fixed with 4% of PFA;
	Anti-Calnexin - ER membrane marker Ab at 1/2,500 dilution; lysates at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-CANX Ab in Hepa1-6 cells at 1/100 dilution; cells were fixed with 4% of PFA;
	Anti-Calnexin - ER membrane marker Ab at 1/2,500 dilution; lysates at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	IHC of human cervix using CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;
	IHC of human pancreas using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;
	IHC of human skin using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;
	IHC of mouse myocardium using CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;
	IHC of mouse eye using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;
	IHC of human skin using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:500/DAB detection;