Anti-CANX

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Catalog #: AB0041
Category: Antibodies
Family: Loading Controls
Source: Goat
Application: WB, IHC (F), IHC (P), IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Alternative names: Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.
Gene Identifier/Accession#: Gene ID: 821, ENSG00000127022
Form: Polyclonal antibody supplied as a 200 or 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum and it is identical to AB4100-200.
Concentration: 2.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.
Antigen Sequence: KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:500-1:5,000
IF	1:50-1:500
IHC (P)	1:200-1:1,000
IHC (F)	1:200-1:1,000

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (38)

Almeida-Reis S, Carvalho A, Dias C, et al. Biomolecules 2024 Feb	PMID: 38540702
Lüningschrör P, Andreska T, Veh A. et al. Dev Cell 2023 Sep	PMID: 37506696
Fernandes J, MSc Thesis, NOVA University of Lisbon, Portugal 2023
Cremer T, Voortman LM, Bos E, et al. EMBO J 2023 Sep	PMID: 37519262
Realinho AM, Boia R, Paiva B, et al. Life Sci 2023 Aug	PMID: 37321535
Amaro A, Sousa D, Sá-Rocha M, et al. Nutrients 2023 Aug	PMID: 37630771
Correia B, Sousa M, Ramalho-Santos J. BioChem 2023
Melo B, Sacramento J, Lavergne J, et al. bioRxiv 2023
Fernandes J, Martins F, Olea E, et al. bioRxiv 2023
Marques, CIR, MSc Thesis, Coimbra University 2013

Images

	IHC of human lymph node using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;
	Immunofluorescence – anti-CANX Ab in Hepa1-6 cells at 1/100 dilution; cells were fixed with 4% of PFA;
	Anti-CANX Ab at 1/2,500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	IHC of human heart using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;
	IHC of human skin using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;
	IHC of mouse retina using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;
	IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;
	IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection;