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Catalog #
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AB0048
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Category
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Antibodies
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Family
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Bacteria
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Source
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Goat
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Application
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WB
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Reactivity
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Bacteria
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Description
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Goat polyclonal antibody to Azurin. This protein is a redox partner for enzymes such as nitrite reductase or arsenite oxidase. It transfers electrons from cytochrome c551 to cytochrome oxidase.
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Alternative names
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azu antibody
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant Burkholderia azurin protein produced in E. coli.
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Antigen Sequence
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MLRKLLVPVASPLILLGPSSAECSVDIQGNDEMQKNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIAHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK
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Specificity
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Detects azurin by Western blot.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:5,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Bacteria
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+++
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ND
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ND
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ND
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ND
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Dagci I, Acar M, Turhan F, et al. J Biotechnol 2024 Aug
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PMID: 39159754
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Kalakenger S, Arslan SY, Turhan F, et al. Mol Biotechnol 2023 Jun
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PMID: 37378861
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Paydarnia N, Khoshtinat Nikkhoi S, Fakhravar A, et al. Mol Biol Rep. 2019 Jun
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PMID: 30937652
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Yildiz M, Askin H. Prep Biochem Biotechnol 2019 Jun
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PMID: 31156029
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Unver Y, Gun BS, Acar M, et al. Prep Biochem Biotechnol 2020 Dec
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PMID: 33346686
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Anti-azurin Ab at 1/2,500 dilution; 100 µg of total protein or 50 ng of recombinant protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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