Anti-CAV1

Catalog #
AB0055
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal to Caveolin-1 – caveolae marker. This scaffolding protein is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression.
Alternative names
CAV1, caveolin, caveolae protein 1, cell growth-inhibiting protein 32 antibody.
Gene Identifier/Accession#
Gene ID: 857, ENSG00000105974
Form
Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human CAV1 produced in E. coli.
Antigen Sequence
MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTH
Specificity
Detects a band of approximately 25 kDa by Western blot in the following cell lysate: hCat, MDCK and 3T3.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000
IF 1:25-1:250
IHC (P) 1:200-1:1,000
IHC (F) 1:200-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

Images

Immunofluorescence – anti-CAV1 Ab - Caveolae Marker in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;
Immunofluorescence – anti-CAV1 Ab - Caveolae Marker in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;
Anti-CAV1 Ab at 1/1,000 dilution; 100 µg lysate per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

400 µg
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