Anti-Rab28

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Catalog #: AB0075
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: RAB28 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking.
Alternative names: N/A
Gene Identifier/Accession#: ENSG00000157869, Gene ID: 9364
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab28 produced in E. coli.
Antigen Sequence: MSETQPLVALVGNKIDLEHMRTVKADKHLRFCQENGFSSHFVSAKTGDSVFLCFQKVAAEILGIKLNKAEIEQSQRVVKADIVNYNQEPLSRTVNPPRSSMCAVQ
Specificity: Detects Rab28 by Western blot in transfected cells with GFP-Rab28.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:250-1:2,000
IF	1:25-1:250

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	ND	ND	+++	ND
Rat	+++	ND	ND	+++	ND
Mouse	+++	ND	ND	+++	ND
Monkey	+++	ND	ND	+++	ND
Canine	+++	ND	ND	+++	ND

Images

	Confocal immunofluorescence of primary RPE cells with Rab28 Ab at 1/100 dilution;
	Anti-Rab28 Ab at 1/1,000 dilution; HEK293 cells transfected with GFP-Rab28; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;