-
Catalog #
-
AB0127
-
Category
-
Antibodies
-
Family
-
TAG-Antibodies
-
Source
-
Goat
-
Application
-
WB
-
Reactivity
-
Transfected cells
-
Description
-
Goat polyclonal antibody to myc epitope tag. c-Myc epitope tag is useful for the labeling and detection of proteins by immunoprecipitation, immunostaining and immunoblotting techniques. Because of its small size, it is unlikely to affect the tagged protein's biochemical properties.
-
Alternative names
-
EQKLISEEDL and MYC antibody.
-
Gene Identifier/Accession#
-
N/A
-
Form
-
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
-
Concentration
-
3.00 mg/ml
-
Isotype
-
IgG
-
Clonality
-
Polyclonal
-
Conjugation
-
Unconjugated
-
Immunogen
-
Purified recombinant peptide EQKLISEEDL corresponding to amino acids 410-419 of Human c-Myc produced in E. coli
-
Antigen Sequence
-
EQKLISEEDLNPEQKLISEEDL
-
Specificity
-
This antibody recognizes recombinant proteins containing EQKLISEEDL epitope tag fused to either amino- or carboxy-terminal of targeted proteins in transfected cells.
-
Storage
-
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
-
Special instructions
-
The antibody solution should be gently mixed before use.
-
|
Application
|
Values
|
|
WB
|
1:500-1:2,000
|
-
|
Sample
|
WB
|
IHC (F)
|
IHC (P)
|
IF
|
ELISA
|
|
Transfected cells
|
+++
|
ND
|
ND
|
ND
|
ND
|