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Catalog #
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AB0134
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Category
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Antibodies
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Family
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Loading Controls
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Source
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Goat
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Application
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WB
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Reactivity
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Human, Rat, Mouse, Monkey, Canine
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Description
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Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
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Alternative names
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TUBA1, TUBA4A antibody.
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Gene Identifier/Accession#
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Gene ID: 7277, ENSG00000127824
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Form
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Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant peptide derived from within residues 80 aa to the N-terminus of human alpha tubulin produced in E. coli.
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Antigen Sequence
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MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEI
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Specificity
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This antibody gives a positive signal in the following human (HeLa), rat (NRK), mouse (AtT-20), dog (MDCK) whole cell lysates.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use.
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Application
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Values
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WB
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1:250-1:2,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Human
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+++
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ND
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ND
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ND
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ND
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Rat
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+++
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ND
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ND
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ND
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ND
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Mouse
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+++
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ND
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ND
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ND
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ND
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Canine
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+++
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ND
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ND
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ND
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ND
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Monkey
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+++
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ND
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ND
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ND
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ND
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Anti-Tubulin alpha4A Ab at 1/1,000 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
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