Anti-Ubiquitin
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Catalog #
AB0144
Category
Antibodies
Family
Cancer
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to Ubiquitin. Ubiquitin is a highly conserved of about 8.5 kDa regulatory protein expressed in all eukaryotic tissues. Its function is labelling of proteins for degradation through ubiquitin proteasome system. In order to perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and a complex of degradative enzymes then recognizes the ubiquitinated complex.
Alternative names
HMG20, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, antibody.
Gene Identifier/Accession#
ENSG00000221983
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant human ubiquitin produced in E. coli.
Antigen Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Specificity
Using the recombinant human ubiquitin gives a positive signal by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-Ub Ab at 2,500 dilution; cells stimulated with MG132; lysate at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

300 €

Quantity / Unit Size

600 µg
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