Anti-Human IgG
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Catalog #
AB0147
Category
Antibodies
Family
Secondary Antibodies
Source
Goat
Application
WB, ELISA
Reactivity
Human
Description
Goat polyclonal antibody to human IgG. Immunoglobulin G (IgG) is a monomeric antibody composed of two heavy chains and two light chains and is the most abundant immunoglobulin in blood and extracellular fluids. IgG plays an important role in immune defence by binding antigens from pathogens and contributing to complement activation, opsonization, antibody-dependent cellular cytotoxicity and neutralization of toxins and infectious agents. This antibody is raised in goat against human IgG and, when conjugated, is suitable for the detection of human IgG in applications such as WB, ELISA, IF and IHC. Goat anti-human IgG antibodies are commonly used in immunoassays for the detection, quantification or localization of human immunoglobulins in biological samples.
Alternative names
Immunoglobulin G antibody.
Gene Identifier/Accession#
N/A
Form
Unconjugated polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Full human IgG affinity purified from serum of four normal donors
Antigen Sequence
N/A
Specificity
Under reducing conditions, the antibody detects bands corresponding to human IgG heavy and light chains.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
ELISA 1:20,000-1:100,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND

Images

Anti-IgG Ab at 1:2,500 dilution; 10 µl of human serum per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

800 µg
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