Anti-Nucleolin
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Catalog #
AB0291
Category
Antibodies
Family
Cancer
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to Nucleolin. NCL is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is found associated with pre-ribosomal particles and intranucleolar chromatin. May play a role in the process of transcriptional elongation and chromatin decondensation by binding to histone H1.
Alternative names
B530004O11Rik, C23, D0Nds28, D1Nds28, Nucl antibody.
Gene Identifier/Accession#
ENSG00000115053, Gene ID: 4691
Form
​Polyclonal antibody supplied as a 100 µl (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
1.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.
Antigen Sequence
TLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Specificity
Detects endogenous levels of NCL by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:1,000-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-NCL Ab at 1/2,500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

100 µg
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