Anti-TUBB
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Catalog #
AB0303
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to TUBB. Tubulin beta class I protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. This protein is involved in cortical dysplasia and other, brain malformations.
Alternative names
CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, tubulin beta class I, TUBB5 antibody.
Gene Identifier/Accession#
ENSG00000196230, Gene ID: 203068
Form
Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Recombinant peptide derived from within residues 345 aa to the C-terminus of human TUBB var1 produced in E. coli.
Antigen Sequence
MKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA
Specificity
Detects endogenous levels of all variants (var1 52 KDa, var2 49.5 KDa, var3 27.3 KDa, var4 44.8KDa, var5 41.7 KDa, var6 41.7 KDa) of TUBB in different cell lines by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-TUBB Ab at 1/1,000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

200 µg
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