Anti-rpoD
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Catalog #
AB0316
Category
Antibodies
Family
Bacteria
Source
Goat
Application
WB
Reactivity
Burkholderia, Escherichia, Pseudomonas
Description
Goat polyclonal antibody to RNA polymerase sigma factor rpoD. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation. rpoD is the primary sigma factor during exponential growth. Preferentially transcribes genes associated with fast growth, such as ribosomal operons, other protein-synthesis related genes, rRNA- and tRNA-encoding genes and prfB.
Alternative names
alt, ECK3057 antibody.
Gene Identifier/Accession#
Gene ID: 947567
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Recombinant peptide derived from within residues 330 aa to the C-terminus of rpoD (Escherichia) produced in E. coli.
Antigen Sequence
LHDVSEEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
Specificity
Detects endogenous levels of rpoD by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Escherichia ++ ND ND ND ND
Pseudomonas +++ ND ND ND ND
Burkholderia ++ ND ND ND ND

Images

Anti-rpoD Ab at 1/1,000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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