Anti-OCLN
- Catalog #
- AB0333
- Category
- Antibodies
- Family
- Organelle Markers
- Source
- Goat
- Application
- WB, IHC (F), IHC (P)
- Reactivity
- Human, Rat, Mouse, Monkey, Canine
- Description
- Goat polyclonal antibody to Occludin. OCLN is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier.
- Alternative names
- BLCPMG, Occludin, PTORCH1, PPP1R115 antibody.
- Gene Identifier/Accession#
- ENSG00000197822, Gene ID: 100506658
- Form
- Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 3.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Peptide derived from within residues 500 aa to the C-terminus of human Occludin.
- Antigen Sequence
- CKQLKSKLSHIKKMVGDYDRQKT
- Specificity
- Detects endogenous levels of OCLN in MCR, IMCD3, COS7, MDCK, HUH, HEK293, MCF7, mouse brain by Western blot.
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use.
-
Application Values WB 1:500-1:2,000 IHC (F) 1:250-1:1,000 IHC (P) 1:250-1:1,000 -
Sample WB IHC (F) IHC (P) IF ELISA Human +++ +++ +++ ND ND Rat +++ +++ +++ ND ND Mouse +++ +++ +++ ND ND Canine +++ +++ +++ ND ND Monkey +++ +++ +++ ND ND -
IHC of human appendix using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection. 
Anti-OCLN Ab at 1/1,000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution; 
IHC of mouse stomach using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection. 
IHC of mouse stomach using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection. 
IHC of human pancreas using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection. 
IHC of rat adipose tissue using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection. 
IHC of mouse lung using anti-OCLN antibody and FFPE tissue after heat-induced antigen retrieval. Anti-OCLN Ab at 1:500/DAB detection.
Usage
Reactivity Chart
Images
320 €
Quantity / Unit Size
300 µg
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