Anti-BCL2

Catalog #
AB0378
Category
Antibodies
Family
Cancer
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to BCL2. This protein belongs to a family that act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases.
Alternative names
Apoptosis Regulator Bcl-2, BCL2 Apoptosis Regulator, B-Cell CLL/Lymphoma 2, PPP1R50, Protein Phosphatase 1 Regulatory Subunit 50 antibody
Gene Identifier/Accession#
Gene ID: 596, ENSG00000171791
Form
Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Recombinant affinity purified peptide derived from within residues 143 aa to the N-terminus of human BCL2 produced in E. coli.
Antigen Sequence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVN
Specificity
Detects a band of approximately 25 kDa by Western blot in the following cell lysate: LS174T, Jurkat and HUH.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Monkey +++ ND ND ND ND
Canine +++ ND ND ND ND

Images

Anti-BCL2 Ab at 1:1,000 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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