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Catalog #
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AB0378
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Category
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Antibodies
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Family
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Cancer
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Source
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Goat
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Application
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WB
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Reactivity
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Human, Rat, Mouse, Monkey, Canine
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Description
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Goat polyclonal antibody to BCL2. This protein belongs to a family that act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases.
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Alternative names
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Apoptosis Regulator Bcl-2, BCL2 Apoptosis Regulator, B-Cell CLL/Lymphoma 2, PPP1R50, Protein Phosphatase 1 Regulatory Subunit 50 antibody
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Gene Identifier/Accession#
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Gene ID: 596, ENSG00000171791
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Form
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Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
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Concentration
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2.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Recombinant affinity purified peptide derived from within residues 143 aa to the N-terminus of human BCL2 produced in E. coli.
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Antigen Sequence
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MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVN
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Specificity
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Detects a band of approximately 25 kDa by Western blot in the following cell lysate: LS174T, Jurkat and HUH.
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:2,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Human
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+++
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ND
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ND
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ND
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ND
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Rat
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+++
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ND
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ND
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ND
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ND
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Mouse
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+++
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ND
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ND
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ND
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ND
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Monkey
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+++
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ND
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ND
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ND
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ND
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Canine
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+++
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ND
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ND
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ND
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ND
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Anti-BCL2 Ab at 1:1,000 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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