Anti-Rab3 Pan
- Catalog #
- AB10032
- Category
- Antibodies
- Family
- Vesicle Trafficking
- Source
- Goat
- Application
- WB, IHC (F), IHC (P), IF
- Reactivity
- Human, Rat, Mouse, Monkey, Canine
- Description
- Goat polyclonal antibody to mouse Rab3. Rab3 belongs to the small GTPase superfamily, Rab family. Rab3 is involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
- Alternative names
- RAB3A, RAB3B, RAB3C, RAB3D, RAS-associated protein RAB3A, ras-related protein Rab-3B, RAS-associated protein RAB3C, RAS-associated protein RAB3D, ras-related protein Rab-3A, ras-related protein Rab-3B, ras-related protein Rab-3C, ras-related protein Rab-3D antibody.
- Gene Identifier/Accession#
- ENSG00000105649
- Form
- Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 3.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab3a produced in E. coli.
- Antigen Sequence
- N/A
- Specificity
- Detects total Rab3 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab3a, GFP-Rab3b, and GFP-Rab3c cds by Western blot.
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use.
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Application Values WB 1:250-1:1,000 IHC (F) 1:250-1:1,500 IHC (P) 1:250-1:1,500 IF 1:50-1:250 -
Sample WB IHC (F) IHC (P) IF ELISA Human +++ +++ +++ +++ ND Rat +++ +++ +++ +++ ND Mouse +++ +++ +++ ND Monkey +++ +++ +++ +++ ND Canine +++ ND ND ND ND -
Immunofluorescence – anti-Rab3 Ab in B6 cells at 1/50 dilution; cells were fixed with methanol; 
Anti-Rab3 Ab at 1/500 dilution; AtT-20 cell line lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Usage
Reactivity Chart
External References (1)
Images
320 €
Quantity / Unit Size
600 µg
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