Anti-mCherry
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Catalog #
AB1340
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IHC (P), IF, IEM
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. mCherry is a ~27 kDa protein that is optimally excited at a 587 nm and has a maximum of emission at 610 nm. It is a basic (constitutively fluorescent) red fluorescent protein and It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
Alternative names
Cherry fluorescent protein, dsRed, red fluorescent protein, tdTomato antibody
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in NaHCO3 100 mM pH 9. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide produced in E. coli.
Antigen Sequence
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Storage
Store at -20 C for long-term storage.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
IEM 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ +++ +++ ND +++

Images

Anti-mCherry Ab at 1/2,500 dilution; 293HEK cells transduced with mCherry Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

400 µg
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