Anti-mLychee
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Catalog #
AB1610
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mLychee. mLychee is a monomeric red fluorescent protein (~26–27 kDa) engineered from coral-derived fluorescent proteins originating from the cnidarian Entacmaea quadricolor. It is designed as a bright and photostable reporter suitable for fusion tagging, live-cell imaging, and multicolor applications. mLychee is a basic (constitutively fluorescent) protein with efficient maturation and stable fluorescence. It has a maximum excitation at 568 nm and a maximum emission at 596 nm. Due to its origin and structural similarity to other red fluorescent proteins, anti-RFP or anti-KillerRed antibodies generally recognize mLychee, while anti-GFP antibodies do not cross-react efficiently. mLychee-tagged proteins can be detected in Western blot at approximately 26–27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibody staining can enhance detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in studies of protein localization, trafficking, and live-cell imaging in mammalian and other model systems.
Alternative names
red fluorescent protein antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence
N/A
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ ND ND ND ND ND

Images

Anti-mLychee Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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