Anti-FusionRed
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Catalog #
AB2887
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to FusionRed. FusionRed is a monomeric red fluorescent protein (~26–27 kDa) engineered from coral-derived fluorescent proteins originating from the sea anemone Entacmaea quadricolor. It is designed as a low-cytotoxicity, bright reporter suitable for fusion tagging, live-cell imaging, and long-term expression studies. FusionRed is a basic (constitutively fluorescent) protein with improved maturation and reduced phototoxicity compared to earlier red fluorescent proteins. It has a maximum excitation at 580 nm and a maximum emission at 608 nm. FusionRed-tagged proteins can be detected in Western blot at approximately 26–27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibody staining can enhance detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in studies of protein localization, trafficking, and live-cell imaging, especially where reduced phototoxicity is important.
Alternative names
FusionRed1, FusionRed2, FusionRed3, Far-Red antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ ND ND ND ND ND

Images

Anti-FusionRed Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 40 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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