Anti-Rab11 Pan

(0) Reviews

(2) References

(0) Validations

(0) Q&A

Catalog #: AB3035
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IHC (F), IHC (P)
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to mouse Rab11. Rab11 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and plays essential roles in vesicle and granule targeting. It has been shown that Rab11 associates with recycling endosomes.
Alternative names: CATX-8, GTP-binding protein YPT3, member of RAS oncogene family, MGC1490, Rab11, RAB11A, RAB11B, RAB11C, Rab25, ras-related protein Rab-11B, RB11A, YL8 antibody.
Gene Identifier/Accession#: ENSG00000103769
Form: Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab11a, Rab11b and Rab11c (Rab25) produced in E. coli.
Antigen Sequence: N/A
Specificity: Detects levels of Rab11 by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab11a, GFP-Rab11b and GFP-Rab11c (Rab25) cds.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:250-1:2,000
IHC (F)	1:100-1:400
IHC (P)	1:100-1:400

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	ND	ND
Rat	+++	+++	+++	ND	ND
Mouse	+++	+++	+++	ND	ND
Canine	+++	+++	+++	ND	ND
Monkey	+++	+++	+++	ND	ND

External References (2)

Deissler HL, Lang GK and Lang GE. Graefes Arch Clin Exp Ophthalmol 2018 Oct.	PMID: 30367290
Deissler HL, Sommer K, Lang GK, et al. Exp Eye Res 2020 Jul	PMID: 32712182

Images

	IHC of human appendix using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	Anti-Rab11 Ab at 1/500 dilution; Hepa cell line lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	IHC of human appendix using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	IHC of rat eye using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	IHC of mouse liver using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	IHC of human myocardium using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	IHC of human placenta using anti-Rab11 pan antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab11 Ab at 1:500/DAB detection;
	Anti-Rab11 antibody IHC staining of human mammary tissue; immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval; antibody concentration 1/200;