Anti-CANX, DyLight®550
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Catalog #
AB37550
Category
Antibodies
Family
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Source
Goat
Application
WB, IHC (F), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker conjugated to DyLight®550. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Alternative names
Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.
Gene Identifier/Accession#
Gene ID: 821, ENSG00000127022
Form
Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
DyLight®550
Immunogen
Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.
Antigen Sequence
KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Specificity
Detects a band of 90 kDa by Western blot whole cell lysates.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:200-1:1,000
IF 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF
Human +++ +++ ND +++
Rat +++ +++ ND +++
Mouse +++ +++ ND +++
Monkey +++ +++ ND +++
Canine +++ +++ ND +++

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Anti-CANX Ab conjugated to DyLight@550 at 1/2,000 dilution using cell lysates at 40 µg per lane;

350 €

Quantity / Unit Size

250 µg
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