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Catalog #
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AB37755
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Category
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Antibodies
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Family
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Loading Controls
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Source
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Goat
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Application
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WB, IHC (P), IF
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Reactivity
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Human, Rat, Mouse, Monkey, Canine
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Description
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Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker conjugated to DyLight® 755. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
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Alternative names
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Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.
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Gene Identifier/Accession#
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Gene ID: 821, ENSG00000127022
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Form
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Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
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Concentration
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2.50 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Dylight®755
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Immunogen
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Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.
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Antigen Sequence
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KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
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Specificity
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Detects a band of 90 kDa by Western blot whole cell lysates.
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:5,000
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IF
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1:50-1:500
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IHC (P)
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1:200-1:1,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Human
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+++
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+++
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ND
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+++
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ND
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ND
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Rat
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+++
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+++
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ND
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+++
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ND
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ND
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Mouse
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+++
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+++
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ND
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+++
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ND
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ND
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Monkey
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+++
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+++
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ND
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+++
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ND
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ND
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Canine
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+++
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+++
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ND
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+++
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ND
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ND
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Anti-CANX Ab conjugated to DyLight@755 at 1/2,500 dilution using cell lysates at 40 µg per lane;
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