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Catalog #
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AB49680
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Category
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Antibodies
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Family
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Loading Controls
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Source
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Goat
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Application
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WB, IHC (F), IF
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Reactivity
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Human, Rat, Mouse, Monkey, Zebrafish, Canine
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Description
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Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase) conjugated to DyLight® 680. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
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Alternative names
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glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
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Gene Identifier/Accession#
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Gene ID: 2597, ENSG00000111640
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Form
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Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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2.50 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Dylight®680
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Immunogen
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Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
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Antigen Sequence
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SVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
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Specificity
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Detects a band of 37 kDa by Western blot.
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:5,000
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IHC (F)
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1:200-1:1,000
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IF
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1:50-1:250
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Human
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+++
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+++
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ND
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+++
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ND
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ND
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Rat
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+++
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+++
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ND
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+++
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ND
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ND
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Mouse
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+++
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+++
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ND
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+++
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ND
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ND
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Zebrafish
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+++
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ND
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ND
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ND
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ND
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ND
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Canine
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+++
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+++
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ND
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+++
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ND
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ND
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Monkey
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+++
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+++
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ND
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+++
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ND
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ND
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Anti-GAPDH Ab conjugated to DyLight®680 (AB49680) at 1:2,000 using lysates at 30 µg per lane;
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