Anti-GAPDH, DyLight®680
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Catalog #
AB49680
Category
Antibodies
Family
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Source
Goat
Application
WB, IHC (F), IF
Reactivity
Human, Rat, Mouse, Monkey, Zebrafish, Canine
Description
Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase) conjugated to DyLight® 680. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Alternative names
glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Gene Identifier/Accession#
Gene ID: 2597, ENSG00000111640
Form
Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Dylight®680
Immunogen
Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
Antigen Sequence
SVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Specificity
Detects a band of 37 kDa by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:200-1:1,000
IF 1:50-1:250

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Human +++ +++ ND +++ ND ND
Rat +++ +++ ND +++ ND ND
Mouse +++ +++ ND +++ ND ND
Zebrafish +++ ND ND ND ND ND
Canine +++ +++ ND +++ ND ND
Monkey +++ +++ ND +++ ND ND

Images

Anti-GAPDH Ab conjugated to DyLight®680 (AB49680) at 1:2,000 using lysates at 30 µg per lane;

350 €

Quantity / Unit Size

250 µg
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