Anti-mWatermelon

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Catalog #: AB6717
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IF
Reactivity: Transfected cells
Description: Goat polyclonal antibody to mWatermelon. mWatermelon is a monomeric fluorescent protein (~27 kDa) engineered from coral-derived fluorescent proteins, originating from the cnidarian Discosoma species, and is widely used as a bright reporter and fusion tag for live-cell imaging and protein tracking. It has a maximum excitation at 504 nm and a maximum emission at 515 nm. Unlike GFP, mWatermelon is derived from coral fluorescent proteins and may show limited or no cross-reactivity with anti-GFP antibodies, depending on sequence similarity; therefore, anti-RFP or specific antibodies may be required for reliable detection. It is suitable for applications such as Western blot, immunofluorescence, immunoprecipitation, and ELISA. In Western blot analysis, mWatermelon-tagged proteins are detected at approximately 27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibodies can enhance signal detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in studies of protein localization, trafficking, and live-cell imaging in mammalian and other model systems.
Alternative names: red fluorescent protein antibody
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: N/A
Antigen Sequence: N/A
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:500-1:5,000
IF	1:50-1:500

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA	IEM
Transfected cells	+++	ND	ND	+++	ND	ND

Images

	Immunofluorescence – anti-mWatermelon Ab using HEK293 cells transduced with mWatermelon-Rab1a; cells were fixed with methanol and anti-mWatermelon at 1/250;
	Anti-mWatermelon Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;