Anti-tdTomato
- Catalog #
- AB8181
- Category
- Antibodies
- Family
- Fluorescent Proteins
- Source
- Goat
- Application
- WB, IHC (F), IHC (P), IF, ELISA
- Reactivity
- Transfected cells
- Description
- Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
- Alternative names
- Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.
- Gene Identifier/Accession#
- N/A
- Form
- Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 3.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Purified recombinant peptide produced in E. coli.
- Antigen Sequence
- MEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
- Specificity
- In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use.
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Application Values WB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500 ELISA ND -
Sample WB IHC (F) IHC (P) IF ELISA Transfected cells +++ +++ +++ +++ ND -
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Immunofluorescence – anti-tdTomato Ab in 293HEK cells transfected with cDNA at 1/50 dilution; cells were fixed with 4% of PFA; 
Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Usage
Reactivity Chart
External References (160)
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320 €
Quantity / Unit Size
600 µg
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