Anti-TagBFP
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Catalog #
AB9459
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to TagBFP (blue fluorescent protein). TagBFP is a monomeric blue fluorescent protein (~26–27 kDa) engineered from coral-derived fluorescent proteins originating from the sea anemone Entacmaea quadricolor, and is widely used as a fusion tag and reporter in live-cell imaging and multicolor experiments. TagBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 2008, derived from Entacmaea quadricolor, and is reported to be a very rapidly maturing monomer with very low acid sensitivity. It has a maximum excitation at ~402 nm and a maximum emission at ~457 nm. Unlike GFP-derived proteins, tagBFP is structurally distinct and is not efficiently recognized by anti-GFP antibodies; detection is typically achieved using anti-BFP or anti-RFP/KillerRed-related antibodies, depending on epitope similarity. It is suitable for applications such as Western blot, immunofluorescence, immunoprecipitation, and ELISA. In Western blot analysis, tagBFP-tagged proteins are detected at approximately 26–27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibody staining can enhance signal detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in multicolor imaging, protein localization, and live-cell studies in mammalian and other model systems.
Alternative names
mTagBFP antibody
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ ND ND ND ND ND

Images

Anti-TagBFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 40 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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