Anti-TUBA4A

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Catalog #: AB0046
Category: Antibodies
Family: Loading Controls
Source: Goat
Application: WB, IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
Alternative names: TUBA1, TUBA4A antibody.
Gene Identifier/Accession#: Gene ID: 7277, ENSG00000127824
Form: Polyclonal antibody supplied as a 200 or 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 2.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.
Antigen Sequence: MYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
Specificity: This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:250-1:2,000
IF	1:50-1:200

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	ND	ND	+++	ND
Rat	+++	ND	ND	+++	ND
Mouse	+++	ND	ND	+++	ND
Canine	+++	ND	ND	+++	ND
Monkey	+++	ND	ND	+++	ND

External References (4)

Goncalves SMM, PhD Thesis, University of Minho, Portugal 2015
Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct.	PMID: 31613922
Saviano A, De Vita S, Chini MG, et al. Biomolecules 2022 Jan	PMID: 35053247
Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar	PMID: 35333565

Images

	Anti-Tubulin alpha4A Ab at 1/1,000 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-Tubulin alpha4A Ab - Microtubule Marker in mouse embryonic fibrolasts at 1/50 dilution;
	Immunofluorescence – anti-Tubulin alpha4A Ab - Microtubule Marker in mouse embryonic fibrolasts at 1/50 dilution;